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首页> 外文期刊>Revista Brasileira de Fruticultura >Avalia??o por RAPD de plantas de abacaxizeiro cultivar Smooth Cayenne derivadas do seccionamento do talo e cultura de tecidos
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Avalia??o por RAPD de plantas de abacaxizeiro cultivar Smooth Cayenne derivadas do seccionamento do talo e cultura de tecidos

机译:菠萝切片茎组织和组织培养的RAPD评价

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Plants of pineapple Smooth Cayenne cultivar were collected from a commercial area, Córrego dos Bois farm, in Canápolis, state of Minas Gerais, to evaluate two different processes of propagation, peduncle division and tissue culture. These same plants were characterized by RAPD analysis. According to the two propagation method, the peduncle division was efficient in seedlings propagation, in quantity as well as in quality, at a short period of time, resulting the same phenotype and genetic characteristics (RAPD analysis) from the matrix descent plants. In the tissue culture method, the quantity of seedlings obtained were not good enough that could prove the use of a sophisticated methodology. Besides loosing 70% of the material, it was necessary to expend a long period of time, at least 18 months to obtain the new seedlings. By RAPD analysis of plants obtained by tissue culture were observed different patterns in some samples, those could be related to somaclonal variation.
机译:从米纳斯吉拉斯州加纳波利斯的商业区Córregodos Bois农场收集了菠萝平滑Cayenne栽培品种的植物,以评估繁殖,花梗分裂和组织培养的两种不同过程。通过RAPD分析表征这些相同的植物。根据两种繁殖方法,花序梗在短时间内有效地促进了幼苗的繁殖,无论在数量还是在质量上,都与基质后代植物产生了相同的表型和遗传特性(RAPD分析)。在组织培养方法中,获得的幼苗数量不足以证明可以使用复杂的方法。除了损失70%的材料外,还需要花费很长时间,至少18个月才能获得新的幼苗。通过RAPD分析,通过组织培养获得的植物在某些样品中观察到不同的模式,这些模式可能与体细胞克隆变异有关。

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