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首页> 外文期刊>Revista Brasileira de Fruticultura >AVALIA??O DE PLANTAS MATRIZES DE ABACAXIZEIRO CULTIVAR SMOOTH CAYENNE UTILIZANDO MARCADORES RAPD E PADR?ES ISOENZIMáTICOS
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AVALIA??O DE PLANTAS MATRIZES DE ABACAXIZEIRO CULTIVAR SMOOTH CAYENNE UTILIZANDO MARCADORES RAPD E PADR?ES ISOENZIMáTICOS

机译:应用RAPD标记和同工酶标准评价菠萝平滑辣椒的基质植物

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Twenty matrix plants of pineapple cultivar Smooth Cayenne were collected from commercial area in the farm Córrego dos Bois, Canápolis city - MG, to evaluate similarity and genotypes patterns by both molecular markers, RAPD and isozymes analysis. Collected matrix plants presented the following characteristics: healthy plants, cylindrical fruits, no fasciation, short peduncle, spineless leaves, boring bubilles and fruit weight between 1,6 to 2,2kg. One hundred primers were tested to analyze RAPD patterns, within them, 43 were efficient to amplify the samples. Distinct patterns were observed among the matrix plants indicating that there is genetic variability. In relation to isozyme profiles, 15 isoenzyme systems were tested but only 8 revealed enzymatic activity, where 5 of them presented low resolution. In spite of this, the 8 systems weren't efficient to differentiate the samples not showing polymorphism
机译:从加拿大加那波利斯市Córregodos Bois农场的商业区收集了二十种菠萝栽培品种Cayenne基质植物,以通过分子标记,RAPD和同工酶分析评估相似性和基因型模式。收集到的基质植物表现出以下特征:健康植物,圆柱形果实,无迷恋,短花序梗,无刺叶,无聊的气泡和果实重量在1.6至2.2千克之间。测试了100个引物以分析RAPD模式,其中有43个有效扩增了样品。在基质植物中观察到不同的模式,表明存在遗传变异。关于同工酶谱,测试了15个同工酶系统,但只有8个显示了酶活性,其中5个显示了低分辨率。尽管如此,这8个系统对区分未显示多态性的样本的效率不高

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