Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal C.A.G.; Carvalho-Castro G.A.; Cottorello A.C.; Leite R.C.; Figueiredo H.C.P.

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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

机译：常规PCR与实时PCR诊断虾WSD的比较分析

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The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

机译：这项研究的目的是使用FAM-BHQ1探针标准化和优化qPCR方案，并比较其对TaqMan qPCR和PCR方法对虾WSD的敏感性。 FAM-BHQ1 qPCR表现出更高的临床敏感性，被证明是检测临床样品中WSSV的可靠选择。

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《Revista de microbiologia》 |2013年第3期|共4页
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Leal C.A.G.; Carvalho-Castro G.A.; Cottorello A.C.; Leite R.C.; Figueiredo H.C.P.;
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中图分类微生物学;
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clinical samplessensitivityreal-time PCRWSSVWSD.;

机译：临床样品敏感性实时PCRWSSVSVWSD。;

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1. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD [J] . A.c. Cottorello, C.a.g. Leal, G.a. Carvalho-castro, Brazilian Journal of Microbiology . 2013,第3期

机译：常规PCR与实时PCR诊断虾WSD的比较分析
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4. Tracking Inoculated Nitrifying Bacteria Strains in Commercial Shrimp Aquaculture Using Real-Time PCR (qPCR) [C] . David J. Drahos, Pornlerd Chanratchakool, Philippe Tacon, International Conference on Recirculating Aquaculture . 2009

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5. Expression analysis of the cold-responsive transcripts CLT-a and CLT-b from Poncirus trifoliata by RT-PCR and real-time PCR. [D] . Robbins, Adriana L. 2004

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6. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD [O] . C.A.G. Leal, G.A. Carvalho-Castro, A.C. Cottorello, 2013

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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

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