Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

机译：常规PCR和实时PCR诊断虾WSD的比较分析

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摘要

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

机译：这项研究的目的是使用FAM-BHQ1探针标准化和优化qPCR方案，并比较其对TaqMan qPCR和PCR方法对虾WSD的敏感性。 FAM-BHQ1 qPCR具有更高的临床敏感性，是检测临床样品中WSSV的可靠替代方法。

著录项

期刊名称 Brazilian Journal of Microbiology
作者
C.A.G. Leal; G.A. Carvalho-Castro; A.C. Cottorello; R.C. Leite; H.C.P. Figueiredo;
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作者单位

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年(卷),期 2013(44),3
年度 2013
页码 901–904
总页数 4
原文格式 PDF
正文语种
中图分类微生物学;
关键词
clinical samples sensitivity real-time PCR WSSV WSD;

机译：临床样品;敏感性;实时PCR;WSSV;WSD;

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专利

1. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD [J] . A.c. Cottorello, C.a.g. Leal, G.a. Carvalho-castro, Brazilian Journal of Microbiology . 2013,第3期

机译：常规PCR与实时PCR诊断虾WSD的比较分析
2. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD [J] . Leal C.A.G., Carvalho-Castro G.A., Cottorello A.C., Revista de microbiologia . 2013,第3期

机译：常规PCR与实时PCR诊断虾WSD的比较分析
3. Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample [J] . Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, BMC Biotechnology . 2020,第1期

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4. Tracking Inoculated Nitrifying Bacteria Strains in Commercial Shrimp Aquaculture Using Real-Time PCR (qPCR) [C] . David J. Drahos, Pornlerd Chanratchakool, Philippe Tacon, International Conference on Recirculating Aquaculture . 2009

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5. Expression analysis of the cold-responsive transcripts CLT-a and CLT-b from Poncirus trifoliata by RT-PCR and real-time PCR. [D] . Robbins, Adriana L. 2004

机译：利用逆转录-聚合酶链反应和实时荧光定量PCR分析三叶草的冷应答转录本CLT-a和CLT-b的表达。
6. Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP conventional PCR nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample [O] . Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, 2020

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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

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