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Mtabolismo antioxidativo para separa??o de lotes de sementes de diferentes graus de homogeneidade

机译:抗氧化代谢可分离不同程度均质的种子

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Th seeds deteriorationprocess usually occurs in lots that had matured too early or where improper stored, in such cases an excessive production ofreactive oxygen species can occur, however, changes caused by these radicals production are not always detectable by seedsquality standard tests. Thus, the aim was to separate heterogeneous bean seeds lots and homogeneous maize seeds lots throughphysiological seed quality standard tests, and relate them to the activity of antioxidant enzymes activity to detect differences inseeds vigor. Three lots of bean and maize seeds were subjected to analysis of viability, vigor and activity of antioxidant enzymes(superoxide dismutase, ascorbate peroxidase and catalase). The physiological standard test allowed classifying the lots ofbeans seeds at different levels of vigor confirming their heterogeneity, however, the analysis of antioxidant enzymes showed nosignificant differences among the lots. As for maize seeds, standard tests did not allow to separate the lots at different levels ofvigor demonstrating their homogeneity, however, the activity of antioxidant enzymes allowed to differentiate them. The analysisof enzyme activity of the antioxidative system is not efficient to differentiate heterogeneous lots of bean seeds, however, it isefficient to detect subtle differences between homogeneous maize seeds lots according vigor.
机译:种子变质过程通常发生在过早成熟或储存不当的批次中,在这种情况下,可能会产生过量的活性氧,但是,种子质量标准测试并不总是能够检测到这些自由基产生的变化。因此,目的是通过生理种子质量标准测试,将异质豆种子批次和均质玉米种子批次分离,并将它们与抗氧化酶活性的活性联系起来,以检测种子活力的差异。对三批豆类和玉米种子进行了抗氧化酶(超氧化物歧化酶,抗坏血酸过氧化物酶和过氧化氢酶)的活力,活力和活性分析。生理标准测试可以对豆子批次进行不同程度的活力分类,以确认它们的异质性,但是,抗氧化酶分析显示批次之间没有显着差异。对于玉米种子,标准测试不允许以不同的活力水平分离批次,以证明其同质性,但是抗氧化剂酶的活性可以区分它们。抗氧化系统的酶活性分析不能有效区分异质豆种子,但是,可以根据活力检测均质玉米种子之间的细微差异。

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