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Production of nzymes by filamentous fungus using sugarcane and sugarcane bagasse as substrate

机译:以甘蔗和甘蔗渣为底物的丝状真菌生产酶

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Th production of enzymes by bioprocesses is a good alternative to add value to agroindustrial waste. Sugarcane bagasse, an abundant and cheap by-product of the sugar industry, was tested as a carbon source for the production of biotechnological interesting enzymes. In this work, fungi were isolated from anatomical parts of sugarcane (root, steam and leaf) and, then, were assessed for enzyme production. The isolated and identified fungi were Fusarium sp., Penicillium sp., Trichoderma auroviride and Cladosporium cladosporioides. Trichoderma auroviride was used for enzyme production (xylanase, invertase and protease) using sugarcane as substrate. Xylanase production (2037 U) by Trichoderma auroviride was higher than invertase and protease production; thus, this enzyme was selected for the further studies. The study of the influence of variables (temperature and stirring intensity) on xylanase production by Trichoderma auroviride, using sugarcane bagasse as substrate, showed that the most favorable xylanase production conditions were observed at 25 °C, without stirring intensity and using saline and Tween for enzyme extraction, which led to a 1980 U xylanase activity.
机译:通过生物过程生产酶是增加农业工业废物价值的很好的选择。经过测试,甘蔗渣是制糖业中一种廉价的丰富副产品,可作为生产生物技术有趣酶的碳源。在这项工作中,从甘蔗的解剖部分(根,蒸汽和叶)中分离出真菌,然后评估其酶的产生。分离和鉴定的真菌是镰刀菌属,青霉属,金木霉属和克拉多孢菌。以甘蔗为底物,将木霉Auroviride用于酶的生产(木聚糖酶,转化酶和蛋白酶)。木霉auroviride产生的木聚糖酶(2037 U)高于转化酶和蛋白酶的产量;因此,选择了该酶用于进一步研究。以甘蔗渣为底物对木霉auroviride生产木聚糖酶的变量(温度和搅拌强度)的影响进行的研究表明,在25°C的条件下观察到了最有利的木聚糖酶生产条件,没有搅拌强度,并且使用盐水和吐温酶提取,导致1980 U木聚糖酶活性。

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