Persistence of the same genetic type of Mycoplasma hyopneumoniae in a closed herd for at least two years

摘要

Two cross-sectional studies were carried out in 2013 and 2015 monitoring forMycoplasma hyopneumoniaepresence in a swine farm. In these studies, the genetic diversity ofM. hyopneumoniaewas assessed in clinical specimens using a Multiple Locus Variable-number tandem repeat Analysis (MLVA) targeting P97 R1, P146 R3 and H4 loci. The samples from August 2015 showed the MLVA profile prevalent in June 2013, therefore it can be concluded that a same genetic type ofM. hyopneumoniaecan persist for at least two years in a closed herd. In addition, the nested PCR reactions implemented in this study showed to be useful for MLVA typing in non-invasive clinical samples.