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The effects of heat and molotov incendiary device fluids on DNA analysis

机译:热和莫洛托夫燃烧装置的流体对DNA分析的影响

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Using the findings from terror attacks such as hand-made incendiary devices, the chemical constituency of the explosive can be elucidated to identify the terrorist group responsible for the attack. It is also possible to clarify the incident by analyzing the DNA from the fingerprint. The purpose of this study is to obtain DNA from the fingerprint found on the explosive at the crime scene and to analyze the thermal effect of the DNA on profiling. Fingerprint samples were put on glass, paper and metal sheet surfaces and were individually exposed to diffrent heat conditions for 3 minutes. A total of 108 samples were subjected to DNA analysis using GlobalFiler PCR Amplification Kit and were typed using the ABI 3130 electrophoresis device. From the samples, complete DNA profile was obtained at 50oC and 90oC on the paper surface and at 50oC on the glass and metal surfaces. On all surfaces and at all temperatures, the gender-identifying locus, amelogenin, was able to be observed. This study, which is conducted for the first time in Turkey, may significantly contribute to the identification of attackers and clarification of the events particularly in terror acts.
机译:利用诸如手工燃烧装置之类的恐怖袭击的发现,可以阐明爆炸物的化学成分,从而确定造成袭击的恐怖集团。还可以通过分析指纹中的DNA来澄清事件。这项研究的目的是从犯罪现场爆炸物上发现的指纹中获取DNA,并分析DNA对轮廓分析的热效应。将指纹样本放在玻璃,纸张和金属片的表面上,并分别置于不同的加热条件下3分钟。使用GlobalFiler PCR扩增试剂盒对总共108个样品进行DNA分析,并使用ABI 3130电泳设备进行分型。从样品中,可以在50oC和90oC的纸张表面以及50oC的玻璃和金属表面上获得完整的DNA分布图。在所有表面上和所有温度下,都可以观察到性别识别基因座釉质生成素。这项研究是在土耳其首次进行的,它可能对识别攻击者和澄清事件特别是在恐怖行为方面做出重大贡献。

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