Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System

摘要

Shrimp waste is an important source of bioactive molecules and it undergoes rapid disintegration which leading to environmental pollution. It is necessary to preserve the material adopting the environmentally safe techniques, prior to recovery of bioactive components such as proteins and carotenoids. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. Hence, a feasible protocol for the recovery of protein from Shrimp Waste has been established. The best conditions of partitioning were achieved using ATPS composed of PEG 4000, ammonium citrate salt, pH8, 1M addition of sodium chloride, and 36.28 Tie line length. The maximum percentage yield of protein extracted from shrimp waste was found to be 74.50%.