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首页> 外文期刊>Redox Biology >Standardization and quality control in quantifying non-enzymatic oxidative protein modifications in relation to ageing and disease: Why is it important and why is it hard?
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Standardization and quality control in quantifying non-enzymatic oxidative protein modifications in relation to ageing and disease: Why is it important and why is it hard?

机译:量化与衰老和疾病相关的非酶氧化蛋白修饰的标准化和质量控制:为什么重要,为什么很难?

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Post-translational modifications (PTM) of proteins determine the activity, stability, specificity, transportability and lifespan of a protein. Some {PTM} are highly specific and regulated involving various enzymatic pathways, but there are other non-enzymatic {PTM} (nePTM), which occur stochastically, depend on the ternary structure of proteins and can be damaging. It is often observed that inactive and abnormal proteins accumulate in old cells and tissues. The nature, site and extent of nePTM give rise to a population of that specific protein with alterations in structure and function ranging from being fully active to totally inactive molecules. Determination of the type and the amount (abundance) of nePTM is essential for establishing connection between specific protein structure and specific biological role. This article summarizes analytical demands for reliable quantification of nePTM, including requirements for the assay performance, standardization and quality control, and points to the difficulties, uncertainties and un-resolved issues.
机译:蛋白质的翻译后修饰(PTM)决定了蛋白质的活性,稳定性,特异性,转运性和寿命。某些{PTM}具有高度特异性,并受各种酶促途径调控,但是还有其他非酶促{PTM}(nePTM),它们随机发生,取决于蛋白质的三元结构,并且可能会造成损害。经常观察到,失活和异常的蛋白质会积聚在旧细胞和组织中。 nePTM的性质,位点和范围会导致该特定蛋白质的群体发生结构和功能变化,从完全活跃的分子到完全失活的分子。 nePTM的类型和数量(丰度)的确定对于在特定蛋白质结构和特定生物学作用之间建立联系至关重要。本文概述了对nePTM进行可靠定量的分析要求,包括对测定性能,标准化和质量控制的要求,并指出了困难,不确定性和未解决的问题。

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