Proximate composition, and total phenols, tannins, flavonoids and saponins, and in vitro ruminal fermentation activity of fenugreek cut at three maturity stages

摘要

Toevaluatetheeffectoffenugreekgrowthstageonitsnbsp;totalphenols(TP),tannins(TT),flavonoids(TF)andsaponins(SAP)contents,nbsp;andthenbsp;impactofTTandSAPoninvitroruminalfermentationactivity,nbsp;fenugreekwascutatvegetative(V),fullflowering(FF)andwell-developpedpods(WDP)andservedforproximatecomposition,TP,TT,TF,SAPdeterminations,andforinvitrogasproductionwith(+PEG)orwithout(-PEG)polyethyleneglycol,andwith(+SAP)orwithout(#8211;SAP)itswaterextractcontainingsaponins.DMwaslow(10.03%)atVandincreased(P0.05)atFF(13.04%)andWDP(28.73%).Therewereaconsistentdecrease(P0.05)inCPandanincrease(P0.05)inNDFandADFasfenugreekmatured.TPdecreased(P0.05)by0.005g/100gDMd-1from1.26to0.88g/100gDM.TFnbsp;decreased(P0.05)nbsp;by0.006g/100gDMd-1from0.77to0.26g/100gDM.TT(0.29-0.33g/100gDM)andSAP(5.79-7.02g/100gDM)tendedtodecrease(P0.05)fromVtoWDP.Potentialgasproduction(As)andrateconstantwerenotdifferent(P0.05)betweenthethreematuritystageswhiletherewasadifference(P0.05)inAsbetween+PEG(35.79ml/200mgDM)and#8211;PEG(31.88ml/200mgDM).TheeffectofPEGadditionwasindependent(P0.05)ofthematuritystage.Gasproductionprofilesfor+SAPand-SAPwerenotadequatelydescribedbytheexponentialmodelswithorwithoutlag.Cumulativegasproductionwasnotdifferent(P0.05)betweenfenugreekcutatVandFFandwasthelowest(P0.05)atWDP.Theremovalofsaponins(-SAP)decreased(P0.05)gasproduction.SAPeffectwassignificant(P0.05)atVandFFnotatWDP.Resultsfromthisstudyindicatedthatthevegetativestageisadequateforcuttingfenugreekforfoodormedicinalusesandatwell-developedpodsgrowthstageforuseasaforage.Theuseoffenugreekinruminantnutritionmayprofitfromin-depthstudiesonimpactoffenugreekgenotypesandtheircultivationconditionsontheirantinutritionalfactors,feedutilizationefficiencyandproductsqualitynbsp;