Genetic diversity and distances of Albanian local sheep breeds using microsatellite markers

摘要

SheepareconsideredasanimportantlivestockspeciesinAlbania.Ananalysisofsixmicrosatellitelociin124unrelatedindividualswasperformedtodefinethegeneticstructureandvariabilityoffourlocalsheepbreeds:#8220;Bardhoka#8221;,#8220;Ruda#8221;,#8220;Recka#8221;and#8220;Shkodrane#8221;.31individualswereselectedpereachbreed.Allelediversity,observedheterozygosity,expectedheterozygosit,GSTestimates,DSgeneticdistancewerecalculated.WrightF-statistics,(FIS,FITandFST)valueswereestimated.nbsp;Atotalof98alleleswerefound.Theaveragenumberofallelesperlocuswas8.15.Withinbreeds,themeannumberofallelesrangedfrom7.7inBardhokato8.8inShkodrane.Meanexpectedheterozygosity(He)rangedfrom0.755inBardhokato0.781inShkodrane.Allelicrichnessvariedfrom7.63inBardhokato8.71inShkodrane.InbreedingforallpopulationisratherhighFIS=0.061rangingfrom-0.011inRudato0.087inShkodrane.Thegeneticdifferentiation,meanFsTwas0.106anddemonstratedthat89.4%oftotalgeneticvariationisduetogeneticdifferentiationwithineachpopulation.Intheneighbor#8211;joiningtreebasedonDSdistance,BardhokaandShkodraneweregroupedtogether,thanRudaandfinallyRecka.nbsp;nbsp;ThePrincipalComponentAnalysis(PCA)analysissupportedtheconclusionsuggestedbydendrogrambasedonDSdistance