The Investigation of the Effects of Enalapril and Losartan on Ghrelin Immunoreactivity in Kidney of Streptozotocin-Induced Diabetic Rats

摘要

Summary Objective: The aim of this study was to examine the effects of enalapril and losartan on ghrelin immunoreactivity in the kidney tissues of rats with streptozotocin (STZ) induced diabetes. Materials and Methods: The study involved 28 Wistar albino rats. The rats were allocated to four groups: control group (n=7), diabetes (DM) group (n=7), DM + enalapril group (n=7) and DM + losartan group (n=7). DM, DM + enalapril and DM + losartan groups were administered a single dose of 50 mg/kg of STZ, intraperitoneally. The rats in the treatment groups were orally administered 5 mg/kg/day of enalapril and 10 mg/kg/day of losartan, starting with the onset of diabetes. At the end of the fourth week of the experiment, rats were decapitated. Kidney tissues collected from the animals were processed by using routine histological techniques. Ghrelin immunoreactivity was determined by avidin-biotin-peroxidase method.Results: Ghrelin immunoreactivity in the distal tubules was moderate (++) in the control group and severe (+++) in the diabetic group. In the distal tubules of the treatment groups, ghrelin immunoreactivity was observed to be moderate (++), similar to the control group.Conclusion: It was determined that enalapril and losartan were effective against ghrelin immunoreactivity in the diabetic rat kidney tissues. Top