Screening of Taxol, an Anticancer Drug produced from Pestaloptiopsis stellata isolated from Ficus infectoria

摘要

A taxol producing fungus from Pestalotiopsis stellata was isolated from Ficus infectoria and screened for the production of Taxol. The Taxol isolated from these fungi is biologically active against cancer cell lines and is also spectroscopically identical to authentic Taxol. In order to lower the price of Taxol and market it more available, a fermentation process involving a microorganism would be the most desirable means of supply. The fungus was identified based on the morphology and spore characterization. Secondary metabolites were extracted using Dichloromethane. Taxol extracted from secondary metabolites was characterized by spectral analysis and chromatographic techniques. The λmax for the authentic Taxol is observed at 274 nm with the absorbance at 3.8nm, similarly λmax for the sample Taxol found at 274 nm range with variation in the absorbance at 1.8nm. Spectral data confirms the presence of alkyl groups attached to the aromatic ring with the side chain containing unsaturation hydrocarbons like alkene/ alkyne groups and High Performance Liquid Chromatography (HPLC) analysis showed the yield of 0.044μg/L amount of Taxol produced from the Pestalotiopsis stellata.The bio prospecting of endophytic fungus Pestalotiopsis stellata isolated from Ficus infectoria is discussed may serve as a potential material for the production of Taxol.