Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

摘要

This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (At AOX 1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX 1a transformant ( pYES 2At AOX 1a) showed cyanide resistant and salicylhydroxamic acid ( SHAM )‐sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae . After exposure to oxidative stress, pYES2At AOX 1a showed better survival and a decrease in reactive oxygen species ( ROS ) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2At AOX 1a sustained growth by regulating GPX 2 and/or TSA 2, and cellular NAD +/ NADH ratio. Thus, the expression of At AOX 1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.