Overexpression of KLHL23 protein from read‐through transcription of PHOSPHO2‐KLHL23 in gastric cancer increases cell proliferation

摘要

Gene fusion, as a prototypical pathognomonic mutation, contributes to genome complexity, and the cis ‐transcription‐induced gene fusions generated by read‐through transcription of adjacent genes have been found to be important for tumor development. We screened read‐through transcription events from stomach adenocarcinoma RNA‐seq data and selected three candidates PHOSPHO2‐KLHL23, RPL17‐C18orf32 , and PRR5‐ARHGAP8 , to assess their biological role in gastric cancer. The expression of all three read‐through fusion transcripts was confirmed in gastric cancer cell lines and paired normal/tumor gastric cancer tissues by real‐time quantitative reverse transcription polymerase chain reaction and their expression was found to be significantly higher in the tumor ( P.