Growth inhibition of Colletotrichum gloeosporioides and Phytophthora capsici by native Mexican Trichoderma strains

摘要

An integrated evaluation to determine the potential ofTrichodermaagainst two important phytopathogens is described in the present research.Phytophthora capsiciandColletotrichum gloeosporioideswere the phytopathogens used in the assay to evaluate the inhibition potential byTrichoderma.Trichoderma harzianum, Trichoderma longibranchiatum, Trichoderma yunnanense, Trichoderma asperellum(T2-10 and T2-31) andTrichodermasp. were evaluated. TheTrichodermastrains showed a percentage of inhibition onP. capsiciandC. gloeosporioidesup to 22.5%.P. capsiciwas mycoparasitized by allTrichodermastrains evaluated and alsoC. gloeosporioides,except when it was evaluated againstT. longibranchiatum. When there was a mycoparasitism, it was 100% over phytopathogen biomass. The production of cellulases and chitinases using sugarcane bagasse and chitin from shrimp shell as unique carbon source were evaluated. The best production of endoglucanase was showed byT. harzianum(40.8?U/g), exoglucanase byTrichodermasp. andT. harzianum(2.9 and 2.5?U/g, respectively), by last the best value of chitinases was 22.1, 20.5 and 20.1?U/g showed byT. asperellum(T2-31),T. longibranchiatumandTrichodermasp., respectively. AllTrichodermastrains evaluated showed to be an excellent potential agent to be used as control pest againstC. gloeosporioidesandP. capsici.