Growth inhibition (in vitro) of Colletotrichum gloeosporioides isolated from cassava (Manihot esculenta) using Trichoderma longibrachiatum

摘要

The growth inhibition of?Colletotrichum gloeosporioides?isolated from cassava was studied?in-vitro?using?Trichoderma longibrachiatum. Both fungi were cultured together on the same Petri plate using three different pairing methods. Inoculation of each fungus on separate Petri plates served as controls. For each pairing method, experiments were conducted in five replicates. Radial growth (cm) of both?C. gloeosporioides?and?T. longibrachiatum?in all Petri plates were measured daily for 7 days. In all the three pairing methods,?T. longibrachiatum?significantly inhibited the growth of?C. gloeosporioides?(P > 0.0001). Growth inhibition of?C. gloeosporioides?by?T. longibrachiatum?was better in ‘inoculating antagonist before pathogen’ than in the other two pairing methods (P = 0.05).?C. gloeosporioides?had significant addition of radial mycelia only between days 1 and 2, as well as days 2 and 3 after pairing (DAP) before contact was made with?T. longibrachiatum.?Addition of mycelia mass of?C. gloeosporioides?slowed down significantly by the day upon contact with?T. longibrachiatum?(P = 0.05, R2?= 0.86). F value for day after pairing (DAP) was also highly significant (P > 0.0001).?T. longibrachiatumcould thus be said to possess probable antagonistic tendency against?C. gloeosporioides.