Protein binding pigment by Bacillus pumilus SF214

摘要

Carotenoids are the most widespread group of naturally occurring pigments. These yellow, orange and red colored molecules are found in both eukaryotes and prokaryotes. In photosynthetic organisms, carotenoids play an essential role in light harvesting, while in non-photosynthetic organisms their principal function is to provide protection against photo-oxidative damages. In order to prepare spores of Bacillus pumilus SF214, sporulation was induced by exhaustion method. Electrophoresis of proteins on polyacrylamide gels (SDS-PAGE) was done using extracted of proteins coating spores, for an aliquot of spores treated with 0.1?M NaOH and to determine the sequence of protein band we used Edman degradation technique and the database (BLASTp) software. Protein extracts of red and white spores showed several differences for what concerns the intensity of some protein bands. Clear increasing in pigment production with the progression of the stationary phase of growth. The analysis of protein band transferred on nitrocellulose membrane using Edman degradation technique showed that the sequence alignment obtained is the homologous N-terminal protein of Bacillus subtilis Tasa. This study suggests that pigmentation in spores represents an additional and maybe alternative protection strategy against oxidative stress. For the first time, this study was performed to describe the correlation between the pigment, protein and spore formation, Which support the proposed application in the field of food or medical production as probiotics, this study was performed to describe the correlation between the pigment, protein and spore formation, Which support the proposed application in the field of food or medical production as probiotics.