Natural occurrence of Clerodendron yellow mosaic virus on Bougainvillea in India

摘要

Bougainvillea (Bougainvillea peruviana) is a common and popular ornamental plant of the family Nyctaginaceae commonly grown in the gardens of India. During a survey in the spring seasons of 2012 and 2013, bougainvillea plants throughout Rajasthan State, India exhibited typical symptoms for a begomovirus infection, including leaf curling and stunted growth (Fig. 1). Total DNAs were extracted from leaf samples of ten symptom-bearing and one symptomless plant, and analysed by PCR using virus component-specific?primers (Wyatt & Brown, 1996). Agarose gel electrophoresis of the PCR products showed amplicons of the expected size (~550 bp) suggesting the presence of DNA-A genomic components in the plants exhibiting symptoms. Efforts to detect DNA-B components and associated satellite molecules were also made by PCR using specific primers, PCRc1/PBL1v2040 (Rojas?et al.,?1993) and beta01/beta02 (Briddon et al., 2002), respectively, but were not successful.