In vitro establishment and maintenance of callus of Taxus wallichiana Zucc. for the production of secondary metabolites

摘要

Callus cultures have been established from needles of aseptically germinated seeds of Taxus wallichiana Zucc. The callus was initiated and maintained on different medium with phytohormones. The study showed that fully defined Gamborgs B5 medium supplemented with 2,4-D (2.0 and 5.0 μM) and Kinetin (0.5 and 1.0 μM) was effective for both initiation and sustained growth of callus tissue whereas woody plant medium was very effective in ascertaining totipotency of the callus by showing organogenesis. The HPLC chromatographic evaluation revealed the presence of taxol (0.8499 %) in callus of the species.