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Long- and Short-Chain Plant-Produced Bacterial N-Acyl-Homoserine Lactones Become Components of Phyllosphere, Rhizosphere, and Soil

机译:长链和短链植物产生的细菌N-酰基-高丝氨酸内酯成为叶圈,根际和土壤的组成部分

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Two N-acyl-homoserine lactone (acyl-HSL) synthase genes, lasI from Pseudomonas aeruginosa and yenI from Yersinia enterocolitica, were introduced into tobacco, individually and in combination. Liquid chromatograph-tandem mass spectrometry and thin-layer chromatography confirmed products of lasI and yenI activity in single and cotransformants. Cotransformants expressing plastid-localized LasI and YenI synthases produced the major acyl-HSLs for each synthase in all tissues tested. Total acyl-HSL signals accumulated in leaf tissue up to 3 pmol/mg of fresh weight, half as much in stem tissue, and approximately 10-fold less in root tissues. Acyl-HSLs were present in aqueous leaf washes from greenhouse-grown transgenic plants. Transgenic lines grown for 14 days under axenic conditions produced detectable levels of acyl-HSLs in root exudates. Ethyl acetate extractions of rhizosphere and nonrhizosphere soil from transgenically grown plants contained active acyl-HSLs, whereas plant-free soil or rhizosphere and nonrhizosphere soil from wild-type plants lacked detectable amounts of acyl-HSLs. This work shows that bioactive acyl-HSLs are exuded from leaves and roots and accumulate in the phytosphere of plants engineered to produce acyl-HSLs. These data further suggest that plants that are bioengineered to synthesize acyl-HSLs can foster beneficial plant-bacteria communications or deter deleterious interactions. Therefore, it is feasible to use bioengineered plants to supplement soils with specific acyl-HSLs to modulate bacterial phenotypes and plant-associated bacterial community structures.
机译:将两个N-酰基-高丝氨酸内酯(酰基-HSL)合酶基因,分别来自铜绿假单胞菌(Pseudomonas aeruginosa)的lasI和来自小肠结肠炎耶尔森氏菌(Yersinia enterocolitica)的JPYI引入烟草中。液相色谱-串联质谱法和薄层色谱法证实了单和共转化体中lasI和yenI活性的产物。表达质体定位的LasI和YenI合酶的共转化子在所有测试的组织中为每种合酶产生了主要的酰基-HSL。在叶片组织中积累的总酰基-HSL信号高达3 pmol / mg鲜重,在茎组织中积累的一半,在根组织中降低约10倍。酰基-HSL存在于温室种植的转基因植物的水洗液中。在缺氧条件下生长14天的转基因品系在根系分泌物中产生可检测水平的酰基-HSL。转基因生长植物的根际和非根际土壤的乙酸乙酯提取物中含有活性酰基-HSL,而野生型植物的无植物土壤或根际和非根际土壤中却缺乏可检测的酰基-HSL。这项工作表明,具有生物活性的酰基-HSL从叶子和根部渗出,并积累在经过工程改造以生产酰基-HSL的植物的植物层中。这些数据进一步表明,经生物工程合成酰基-HSL的植物可以促进有益的植物-细菌通讯或阻止有害的相互作用。因此,使用生物工程植物向土壤中添加特定的酰基-HSL来调节细菌表型和与植物相关的细菌群落结构是可行的。

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