气相色谱-质谱法检测细菌中N-酰基高丝氨酸内酯类信号分子

摘要

建立了N-酰基高丝氨酸内酯类(N-acyl-homoserine lactones,AHLs)群体感应信号分子的气相色谱-质谱( GC - MS)检测方法,并优化了检测条件.在优化条件下,采用选择离子扫描模式(SIM)从细菌Pseudoalteromonas sp.NJ6 -3 -1培养液中检测到3种AHLs(N-己基高丝氨酸内酯(C6- HSL)、N-辛基高丝氨酸内酯(C8- HSL)和N-十四基高丝氨酸内酯(C14- HSL)).与传统的薄层层析-生物传感器(TLC - biosensor)和高效液相色谱法(HPLC)相比,该方法更加简单、高效,只需对培养液样品进行简单处理,即可定性检测样品中的AHLs分子.该方法为从复杂基质中检测和鉴定AHLs信号分子提供了有效手段.%A rapid and sensitive method for the determination of N-acyl-homoserine lactones( AHLs) , a kind of quorum sensing signal molecules in Gram-negative bacteria, was established based on gas chromatography - mass spectrometric technique. The GC - MS conditions for the detection of AHLs were optimized. Under the optimal conditions, three kinds of AHLs, e. g. N-hexanoyl-L-homoserine lactone(C6 -HSL) , N-octanoyl-L-homoserine lactone(C8 -HSL) and N-tetradecanoyl-L-homoserine lactone(C14 - HSL) , were detected in the cultured supernatants of bacteria Pseudoalteromonas sp. NJ6 -3-1 under selected ion monitoring(SIM) mode. Compared with the traditional TLC-biosensor and HPLC method, this method is rapid and simple. AHLs in the samples could be detected qualitatively only by a simple sample handling procedure. The study provides a useful method in the detection of AHLs from complicated mixtures.