Rhizosphere bacterial community PCR-DGGE profiles and metal speciation in shooting range soils

摘要

Chemical immobilization in conjunction with vegetation coverage is a practical remediation technology for metal contaminated soils. This paper evaluated metal solubility and bacterial community structure in a highly contaminated shooting range soil treated with a phosphorus amendment (AP) and plant growth. The low dose of amendment addition decreased the SPLP-Pb concentration to 2.1 mg/L. However, the high dose treatment increased Pb solubility more than that of Control soil. The soils treated with the amendment along with plant growth (Plant + AP) had a lower SPLP-Pb concentration than the soils treated solely with the amendment. The soils with the amendment addition had a decreased number of bands in their DGGE profile, and the detrimental effectof amendment appeared more significant for the high dosage than in low dosage. According to the DNA sequence identification, the band corresponding to Bacillus sp., and Rhizobium sp. that dominated in the Control and Plant soils was not observed in the soil with the high dose of amendment.