Effect of curcumin on the modulation of αA- and αB-crystallin and heat shock protein 70 in selenium-induced cataractogenesis in Wistar rat pups

摘要

Purpose: To investigate the expression of αA- and αB-crystallin and heat shock protein 70 (Hsp 70) during curcumin treatment of selenium-induced cataractogenesis in Wistar rat pups. Methods: Group I Wistar rat pups received only saline and served as the control. Group II Wistar rat pups were intraperitoneally injected with selenium (15 μM/kg bodyweight) to induce cataract. Group III Wistar rat pups also underwent selenium-induced cataract but were cotreated with 75 mg/kg body weight of curcumin (single oral dose). Group IV Wistar rat pups with selenium-induced cataract were post-treated with curcumin at the group III dosage 24 h after selenium administration. Group V Wistar rat pups with selenium-induced cataract were pretreated with curcumin at the group III dosage 24 h before selenium administration. Results: This study found higher levels of αA- and αB-crystallin and Hsp 70 in lenses injected with selenium alone (group II) than in control lenses (group I). Similar results were observed in the group III and IV lenses. In contrast, in group V, the presence of curcumin 24 h before selenium injection decreased the αA- and αB-crystallin and Hsp 70 levels to almost the same as those found in group I lenses. Conclusions: Curcumin suppressed the expression of selenite-induced αA- and αB-crystallin and Hsp 70, and may therefore suppress cataract formation in rat pups.