Bacterial Diversity of Ny-?lesund, Arctic Archipelago Svalbard

摘要

The bacterial diversity of the water sample, collected from Ny-?lesund,Arctic Archipelago Svalbard was analyzed by a phenotypic as well asa genotypic approach. Pure colonies of the culturable bacteria wereestablished and grown at a range of temperatures: 4oC, 15oC, 22oC and 37oC.Optimum growth was found at 15oC, and around 28 colonies were obtained.The library was dominated by 16S rDNAs of Gram-negative bacteria(γ-Proteobacteria). Twenty two isolates exhibited sequences were similar tothat of known bacterial isolates (>97% sequence similarity), represented bythe species of the genera Psychrobacter, Pseudomonas, and Acinetobacter.Six isolates exhibited sequences showed less affi liation with known taxa(<97% sequence similarity), and may represent novel taxa.