Purpose: To investigate changes inprotein profiles of posterior sclera in guinea pigs during developmentof form deprivation myopia and recovery. Methods: Three groups of guinea pigs(developing form deprivation myopia, recovering from the myopia andnormal control) were evaluated for protein profiles of the posteriorsclera using two-dimensional gel electrophoresis. Protein spots with adifferent intensity of at least threefold among the 3 groups werefurther identified with mass spectrometry. Key proteins associated withocular growth (crystallins) were examined at mRNA levels using RT–PCR. Results: Moderate myopia was induced at7 weeks of monocular deprivation and then more gradually recoveredtoward the previous refractive status 4 days after re-exposure of theeye to normal visual conditions. The profile of all protein spots atthe posterior sclera was similar for both the deprived and the recoveryeyes but distinct between either of the 2 experimental eyes and thenormal control eyes. Twenty-six and 33 protein spots weredifferentially expressed in the deprived and the recovery eyes,respectively, compared to the normal control eyes. In contrast, thenumber of proteins differentially expressed between the deprived andthe recovery eyes was only 5. Among the different subtypes ofcrystallins, βB2-crystallin was down-regulated and βA4-crystallin wasupregulated in the deprived eyes at both protein and mRNA levelscompared to the normal control eyes. The trend of expression forβA3/A1-crystallin was also similar at both mRNA and protein levels forthe deprived eyes. However, αA-crystallin mRNA in the recovery eyes wasupregulated while αA-crystallin itself was down-regulated. A similarinconsistency in expression of βA3/A1-, βA4-, and βB2-crystallinsbetween the protein and mRNA levels also occurred in the recovery eyes.Conclusions: Proteomic analysis providesa useful survey of the number of proteins whose levels change duringform deprivation myopia and the subsequent recovery. In particular, thecrystallins changed during the development of form deprivation myopiaand recovery. The changes in crystallin protein levels were consistentwith that in mRNA levels during the development stage ofform-deprivation myopia (FDM). However, the changes of most crystallinprotein levels were mismatched with mRNA levels during the recoverystage.
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