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首页> 外文期刊>Memórias do Instituto Oswaldo Cruz >Distribui??o temporal de perfis eletrofocéticos de Acido nucleico de rotavírus em fezes de crian?as em Belém, Pará
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Distribui??o temporal de perfis eletrofocéticos de Acido nucleico de rotavírus em fezes de crian?as em Belém, Pará

机译:帕拉贝伦儿童粪便中轮状病毒核酸电泳图谱的时间分布

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Electrophoresis of rotavirus RNA in polyacrylamide gel was casrried out by using faecal samples collected from children living in the peripheral area of Belém, Brazil. Typical rotavirus profiles (eleven bands) were noted in 46(76.7%) out of 60 ELISA rotavirus positive specimens. According to the eletrophoretic patterns, 5(10.9%) were classified as subgroup I, while 41(89.1%) of them as subgroup II. Lacking of bands 10 and 11 in 14(23.3%) samples did not allow us to classify their electropherotypes. The profile coded as "1N2L" was the predominant one, ccouting for 65.2% of the classified strains.
机译:轮状病毒RNA在聚丙烯酰胺凝胶中的电泳方法是通过使用收集自巴西贝伦市周边地区儿童的粪便样品进行的。在60个ELISA轮状病毒阳性标本中,有46个(76.7%)记录了典型的轮状病毒谱(11条带)。根据电泳图谱,将5(10.9%)归为I亚组,将其中41(89.1%)归为II亚组。 14个样本(23.3%)中缺少10和11谱带,因此无法对它们的电电泳类型进行分类。编码为“ 1N2L”的图谱是最主要的图谱,占分类菌株的65.2%。

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