PROCEDIMIENTO PARA LA AMPLIFICACION, EXENTA DE TRANSCRIPCIONES, DE ACIDOS NUCLEICOS;APLICACION DE DICHO PROCEDIMIENTO PARA EL DIAGNOSTICO DE ENFERMEDADESEN FASE EXTERNA EN EL HOMBRE Y ANIMALES;Y UN EQUIPO TRANSPORTABLE DE REACTIVOS PARA REALIZAR DICHO PROCEDIMIENTO.

摘要

PCT No. PCT/AT96/00106 Sec. 371 Date Apr. 6, 1998 Sec. 102(e) Date Apr. 6, 1998 PCT Filed Jun. 13, 1996 PCT Pub. No. WO97/00330 PCT Pub. Date Jan. 3, 1997The present invention relates to a process for the transcriptionless amplification of nucleic acids or nucleic acid sequences by means of enzymes in which the nucleic acids or sequences are divided at least partially into their individual strands before amplification and/or transcribed and the reaction mixture contains oligonucleotides with a base sequence essentially complementary to those of the ends of the nucleic acids or sequences to be amplified, where said oligonucleotides can, in certain conditions, form starting points and/or chemical components for the synthesis of nucleic acids and are built into the amplification product to be formed. The product of the components itself again corresponds to the nucleic acid or sequence to be amplified. The reaction product formed from the oligonucleotide components, possibly from the templates and other chemical components, reacts again in one step with the oligonucleotide components, the reaction being conducted substantially isothermally.