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首页> 外文期刊>Memórias do Instituto Oswaldo Cruz >Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
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Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting

机译:在高负担情况下实验室实时诊断中使用室内实时聚合酶链反应鉴定结核分枝杆菌分离株的性能

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摘要

Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA- hsp 65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA- hsp 65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
机译:巴西是结核病高负担的国家之一,迅速诊断对于有效控制该疾病至关重要。在本研究中,在参考实验室的常规诊断条件下,评估了针对mpt64基因的内部实时聚合酶链反应(PCR)分析,以鉴定结核分枝杆菌复合体。从2011年5月至2012年7月,前瞻性提交了1520株分枝杆菌,用于表型和/或PRA-hsp 65的鉴定和实时PCR。 mpt64实时PCR显示99.7%的敏感性和96%的特异性,检测到79.4%的表型和PRA-hsp 65鉴定漏诊的病例。内部实时PCR检测显示出高灵敏度和特异性,并成功地在高负担的参考实验室中对参考实验室的结核病进行了常规诊断。

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