BackgroundThe degree to which host genetic variation can modulate microbial communities in humans remains an open question. Here, we performed a genetic mapping study of the microbiome in two accessible upper airway sites, the nasopharynx and the nasal vestibule, during two seasons in 144 adult members of a founder population of European decent. ResultsWe estimated the relative abundances (RAs) of genus level bacteria from 16S rRNA gene sequences and examined associations with 148,653 genetic variants (linkage disequilibrium [LD] r 2 q Dermacoccus (phylum Actinobacteria) and a variant 8?kb upstream of TINCR (rs117042385; p =?1.61?×?10?8; q =?0.002), a long non-coding RNA that binds to peptidoglycan recognition protein 3 ( PGLYRP3 ) mRNA , a gene encoding a known antimicrobial protein. A second association was between a missense variant in PGLYRP4 (rs3006458) and the RA of an unclassified genus of family Micrococcaceae (phylum Actinobacteria) ( p =?5.10?×?10?7; q?= 0.032). ConclusionsOur findings provide evidence of host genetic influences on upper airway microbial composition in humans and implicate mucosal immunity genes in this relationship.
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机译:背景宿主遗传变异可以调节人类微生物群落的程度仍然是一个悬而未决的问题。在这里,我们在两个体面的欧洲体面创始人群的144个成年成员中,在两个季节中对鼻咽和鼻前庭进行了微生物组的遗传图谱研究。结果我们从16S rRNA基因序列估计了属水平细菌的相对丰度(RAs),并检查了与148,653个遗传变异(连锁不平衡[LD] r 2 sup> q皮球菌(门放线菌)和8个变异)的关联。 TINCR上游kb(rs117042385; p =?1.61?×?10 ?8 sup>; q =?0.002),这是一种长的非编码RNA,与肽聚糖识别蛋白3(PGLYRP3)mRNA结合, PGLYRP4的一个错义变异(rs3006458)与一个未分类的微球菌科(Actinobacteria)的RA之间的第二个关联(p =?5.10?×?10 ?7 sup>; q?= 0.032)。结论我们的发现为宿主遗传因素对人类上呼吸道微生物组成的影响提供了证据,并在这种关系中暗示了粘膜免疫基因。
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