Serum ROCK1 mRNA is of great diagnostic value for glioma patients

摘要

The study aimed to measure the presence of rho-associated protein kinase 1 ( ROCK1 ) mRNA in serum samples collected from glioma and investigate its diagnostic significance in glioma . The presence of ROCK1 mRNA was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between ROCK1 mRNA and clinical characteristics was analyzed via Chi-square test. The criteria of diagnosis evaluation, including sensitivity, specificity, optimal cutoff point, and area under the curve (AUC) were determined through the receiver operating characteristic ( ROC ) curve analysis. ROCK1 mRNA was significantly increased in serum samples collected from glioma patients compared to the controls ( P .05). In addition, the optimal cutoff point was 3.025, with the sensitivity and specificity of 88.89% and 79.25%, respectively. The AUC was 0.881, indicating that ROCK1 was a diagnostic biomarker for glioma patients ( P .0001, 95% CI = 0.829–0.933). Serum ROCK1 mRNA is significantly up-regulated in glioma cases compared to healthy controls. ROCK1 may be a potential diagnostic biomarker in glioma .