Striatal Tyrosine Hydroxylase Is Stimulated via TAAR1 by 3-Iodothyronamine, But Not by Tyramine or β-Phenylethylamine

摘要

The trace amine-associated receptor 1 (TAAR1) is expressed by dopaminergic neurons, but the precise influence of trace amines upon their functional activity remains to be fully characterized. Here, we examined the regulation of tyrosine hydroxylase (TH) by tyramine and beta-phenylethylamine (β-PEA) compared to 3-iodothyronamine (T_(1)AM). Immunoblotting and amperometry were performed in dorsal striatal slices from wild-type (WT) and TAAR1 knockout (KO) mice. T_(1)AM increased TH phosphorylation at both Ser~(19)and Ser~(40), actions that should promote functional activity of TH. Indeed, HPLC data revealed higher rates of L -dihydroxyphenylalanine (DOPA) accumulation in WT animals treated with T_(1)AM after the administration of a DOPA decarboxylase inhibitor. These effects were abolished both in TAAR1 KO mice and by the TAAR1 antagonist, EPPTB. Further, they were specific inasmuch as Ser~(845)phosphorylation of the post-synaptic GluA1 AMPAR subunit was unaffected. The effects of T_(1)AM on TH phosphorylation at both Ser~(19)(CamKII-targeted), and Ser~(40)(PKA-phosphorylated) were inhibited by KN-92 and H-89, inhibitors of CamKII and PKA respectively. Conversely, there was no effect of an EPAC analog, 8-CPT-2Me-cAMP, on TH phosphorylation. In line with these data, T_(1)AM increased evoked striatal dopamine release in TAAR1 WT mice, an action blunted in TAAR1 KO mice and by EPPTB. Mass spectrometry imaging revealed no endogenous T_(1)AM in the brain, but detected T_(1)AM in several brain areas upon systemic administration in both WT and TAAR1 KO mice. In contrast to T_(1)AM, tyramine decreased the phosphorylation of Ser~(40)-TH, while increasing Ser~(845)-GluA1 phosphorylation, actions that were not blocked in TAAR1 KO mice. Likewise, β-PEA reduced Ser~(40)-TH and tended to promote Ser~(845)-GluA1 phosphorylation. The D_(1)receptor antagonist SCH23390 blocked tyramine-induced Ser~(845)-GluA1 phosphorylation, but had no effect on tyramine- or β-PEA-induced Ser~(40)-TH phosphorylation. In conclusion, by intracellular cascades involving CaMKII and PKA, T_(1)AM, but not tyramine and β-PEA, acts via TAAR1 to promote the phosphorylation and functional activity of TH in the dorsal striatum, supporting a modulatory influence on dopamine transmission.