Plasma Lipidomic Signature of Rectal Adenocarcinoma Reveals Potential Biomarkers

摘要

Background Rectal adenocarcinoma (RAC) is a common malignant tumor of the digestive tract and survival is highly dependent upon stage of disease at diagnosis. Lipidomic strategy can be used to identify potential biomarkers for establishing early diagnosis or therapeutic programs for RAC. Objective To evaluate the lipoperoxidation biomarkers and lipidomic signature in the plasma of patients with RAC ( n ?=?23) and healthy controls ( n ?=?18). Methods Lipoperoxidation was evaluated based on malondialdehyde (MDA) and F_(2)-isoprostane levels and the lipidomic profile obtained by gas chromatography and high resolution mass spectrometry (ESI-q-TOF) associated with a multivariate statistical technique. Results The most abundant ions identified in the RAC patients were those of protonated phosphatidylcholine and phosphatidylethanolamine. It was found that a lisophosphatidylcholine (LPC) plasmalogen containing palmitoleic acid [LPC (P-16:1)], with highest variable importance projection score, showed a tendency to be lower in the cancer patients. A reduction of n?? ?3 polyunsaturated fatty acids was observed in the plasma of these patients. MDA levels were higher in patients with advanced cancer (stages III/IV) than in the early stages groups and the healthy group ( p ?