Erratum: An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis

摘要

Reason for Erratum: Due to a typesetting error, a misalignment in Table 2 lead to the publication of incorrect information. In the “Resolving gel” column, line “1.5M TRIS-HCl, pH 8.8,” the volume should be 4.5 mL, and not 4.5 L as published. Table 2 Detailed protocol for gel and electrophoresis experiments . Stacking gel Resolving gel 10X Running Buffer Light gel Heavy gel Moving solution Conservation buffer DTT solution Iodoacetamine solution Equilibration buffer 5X running buffer Overlays solution 40% acrylamide/bisac rylamide (37.5:1) (w/v) 1 mL 6.3 mL 1.5M TRIS-HCl, pH 8.8 4.5 mL 52.5 mL 52.5 mL 50 mL 100 mL 50 mL 0.5M TRIS-HCl, pH 6.8 2.5 mL 10% SDS (w/v) 0.1 mL 0.18 mL 2.1 mL 2.1 mL 4 mL 10% APS 0.05 mL 0.09 mL 2.1 mL 1.05 mL TEMED 0.015 mL 0.009 mL 360 μL 60 μL Glycine 120 g SDS 40 g 20 g 40 g TRIS 576 g 60.5 g 30% acrylamide/bisac rylamide (37.5:1) (w/v) 68 mL 128 mL Glycerol 18 mL 74 g 378 g 1% bromophenol blue 210 μL 20 μL 1M DTT 0.1 g Iodoacetamine 1.12 g Equilibration buffer 10 mL 25 mL Urea 355 g Agarose type I-A 0.4 g Agarose type VII 0.1 g 5X running buffer 20 mL Apyrogenic water 6.35 mL 6.93 mL to 4L 85 mL 14 mL 90 mL 296 mL To 1L to 4L 80 mL Acrylamide/bisacrylamide (37.5 :1) solution (w/v) (BioBasic Inc, Markham, Ontario, Canada), agarose type I-A (Sigma, Oakville, Québec, Canada), ammonium persulfate (BioBasic Inc, Markham, Ontario, Canada), bromophenol blue (Sigma, Oakville, QC, Canada), dithiothreitol (DTT) (BioBasic Inc, Markham, Ontario, Canada), glycerol (BioBasic Inc, Markham, Ontario, Canada), glycine (BioBasic Inc, Markham, Ontario, Canada), iodoacetamide (BioBasic Inc, Markham, Ontario, Canada), sodium dodecyl sulfate (SDS) (BioBasic Inc, Markham, Ontario, Canada), TRIS (BioBasic Inc, Markham, Ontario, Canada), TRIS-HCl (BioBasic Inc, Markham, Ontario, Canada), tetramethylethylenediamine (TEMED) (BioBasic Inc, Markham, Ontario, Canada), urea (BioBasic Inc, Markham, Ontario, Canada), 2- hydroxythylagarose type VII (Sigma, Oakville, Québec, Canada). The publisher apologizes for this error and the correct version of Table 2 appears below. This error does not change the scientific conclusions of the article in any way.