Evaluating the persistence of Phlebiopsis gigantea (Fr.: Fr.) Jülich mycelium and fruiting bodies in pine stumps after root-rot protection treatments

摘要

Commercial preparations Rotstop and PgSuspension, used in Scandinavia and the United Kingdom, are EUlicensedbiocontrol agents against root rot that contain isolates of the fungus Phlebiopsis gigantea. The composition ofthese two products differs from that of PgIBL, previously use in Poland, hence the need to verify their effectivenessunder Polish site and stand conditions. Stumps were treated with the commercial products and subsequently checkedafter one year for the persistence of mycelia and fruiting bodies. Molecular methods were used on the mycelia presentin the stumps to confirm that their genetic identity was consistent with fungal isolates from the preparations.Treatments with EU-licensed preparations gave satisfactory results – in about 70–80% of the stumps mycelia werepresent under the bark. Inoculation with Rotstop preparations was most effective during autumn 2008 (effectivenessof 82%), while PgSuspension application was most effective during spring 2008 (effectiveness of 77%).The development of the mycelia in pine stumps from the two preparations investigated (containing preserved dormantspores) was similar to that of preparation PgIBL (formulated from living mycelium within a growing medium), in termsof their relative effectiveness measured as the percentage of stumps supporting under-bark mycelia (which rangedbetween 70–90%, depending on the treatment term).