Characterization of Virulence Genes Present in Corynebacterium pseudotuberculosis Strains Isolated From Buffaloes

摘要

Forty aspirates collected from swellings of buffaloes showing clinical symptoms of the OedematousSkin Disease were examined bacteriologically, biochemically and by uniplex and multiplex PCR for virulencegenes screening. Ten isolates were identified as Corynebacterium pseudotuberculosis in a percentage of (25%)and all isolates exhibited a synergistic hemolytic activity with Rhodococcus equi culture filtrate andinhibited staphylococcal hemolytic activity. The presence of universal 16s gene of C. pseudotuberculosis wasconfirmed by uniplex PCR in all 10 isolates. Application of multiplex PCR was done and all isolates ofC. pseudotuberculosis were positive for presence of pld gene of Corynebacterium pseudotuberculosis whileonly 5 isolates were positive for presence of pld gene of Corynebacterium ulcerans and 7 isolates werepositive for presence of diphtheria toxin dt gene of Corynebacterium ulcerans.