Single Cell Sexing Using a Part of Metal Razor Blade of Bovine Embryos Through Male Specific Repetitive DNA

摘要

The development of quick and sensitive methods for sexing of bovine embryos in early stage wouldbe considerable in economic advantages. Polymerase Chain Reaction (PCR) were used for amplification of DNAsequenced derived from the male sex chromosome. The sexed method was allows to removed single cell biopsyusing a part of metal razor blade from bovine embryos in less than two minutes without compromisedevelopmental potential. The embryo biopsy can be used for male sex determination. Definite signals followingPCR amplification were obtained in all cases indicated that single blastomere cell from preimplantation of bovineembryos was sufficient for male sex determination. Male sex determination of the embryos was developedfurther in both in vitro and in vivo successfully.