Evidences for Diagnosis of Feline Panleucopenia Viral Infection in Cats in Egypt: Molecular, Pathological and Histopathological Findings

摘要

Fifty cats of different of ages, sex, breeds naturally infected with Feline Panleucopenia virus (FPV),four healthy control cats. All 50 cats hospitalized for supportive & palliative treatment .Each cat was examinedclinically to detect clinical manifestations of the disease showing symptoms suggestive for FP as well as ELISA,gross pathology, histopathology and PCR amplification analysis were conducted. 1-clinical signs detected inall diseased cats in the form of lethargy, fever, anorexia, thirst, diarrhea, vomiting and dehydration. 2-RapidELISA snip test for qualitative detection of viral antigen were positive when carried out on feces of infectedcats and negative for the control one. The prognosis was bad and all diseased cats were died. 3- Postmortemexamination showed dehydration, vomiting, diarrhea of offensive odour, Jejunum and ilium filled with gases,were odematous with petechial hemorrhage on both mucosa and serosal surfaces, mesenteric lymph nodes werehemorrhagic and odematous. Liver & spleen enlarged and severely congested. 4- Histopathological findingson intestine villi showed sever apical necrosis with atrophy segmental loss of crypts, lamina pro4pia werecollapsed contained sloughed epithelium, debris and fibrin, severely infiltrated with inflammatory cell insubmucosal area. Mesenteric lymph nodes, follicles showed focal necrosis, sever sinusoidal congestion andsome of them contain fibrin. 5- PCR amplification analysis were conducted , revealed that the amplificationproducts from tissue samples (Jejunum, ilium and mesenteric lymph nodes) were confirmed as FPV (VP1) geneby nucleotides sequence analysis presented in database.