Detection of Aerolysin, Hemolysin Genes and Antimicrobial Susceptibility of Aeromonas hydrophila Isolated from Retail Foods and Human Stool Samples

摘要

Aeromonads are ubiquitous in aquatic environment and considered as an emerging food andwater-pathogen constituting a public health threat to the consumers particularly in the under-developingcountries mainly for young and immunocompromised persons. So a total of 265 food and human stool sampleswere examined to detect the presence of Aeromonas hydrophila and their virulence genes and inquest theirantimicrobial susceptibility pattern. A. hydrophila was recovered from 15.3% of the human fecal samples(17.5% from diarrheic and 14.5% from non-diarrheic stool), as well 20 % of minced meat, 25 % of shrimps and30 % of fish. Isolates were represented to be A. hydrophila depending on morphological, microscopic andbiochemical tests. Moreover isolates were subjected to polymerase chain reaction (PCR) technique for detectionof Aero and Hly genes, responsible for aerolysin and hemolysin toxin production in the isolates, only oneisolate represented Aerogene. Antibiotic susceptibility test implied that the isolates show maximum (100%)sensitivity to ciprofloxacin and gentamycin followed by tetracycline and streptomycin respectively and 100%resistance to penicillin, ampicillin and colistin. Isolation of A. hydrophila from a variety of retail foods andhuman samples, as well as their resistance to most of the commercial antibiotics used necessitates that foodstuffs should be monitored carefully as a possible source of food-borne infection.