对分离自发病宽体金线蛭（ Whitmania pigra）、中国林蛙（ Rana chensinensis）、牙鲆（ Paralichthys olivaceus）、草鱼（ Ctenopharyngodon idellua）、青鱼（ Mylopharyngodon piceus）、鲤（ Cyprinus carpio）的42株致病性嗜水气单胞菌（Aeromonas hydrophila）采用PCR方法，进行了溶血素（Ahh）基因的检测，并将代表菌株的Ahh基因片段通过Blast与GenBank中报道的相似性较高的10株嗜水气单胞菌参考菌株进行了核苷酸同源性分析。结果显示，所检不同来源的嗜水气单胞菌均携带Ahh基因；所测代表菌株Ahh基因片段与10株嗜水气单胞菌参考菌株的同源性为94.1％～98.9％；供试不同来源嗜水气单胞菌株间Ahh基因的同源性为97.1％～100％，其中分离自草鱼的HC960715-5与分离自宽体金线蛭的HTQ010505-3之间同源性高达100％，分离自青鱼的HL060811-3与分离自林蛙的HQ070610 B-3同源性也高达100％。%Forty-two pathogenic Aeromonas hydrophila strains isolated from Whitmania pigra, Rana chensinensis, Parali-chthys olivaceus, Ctenopharyngodon idellua, Mylopharyngodon piceus and Cyprinus carpio were investigated for the pres-ence of hemolysin ( Ahh) gene using PCR method , and the homology of Ahh gene sequences from diverse representative A.hydrophila strains were compared with available sequences in the NCBI GenBank using the soft -ware DNAStar.The re-sults showed that all the detected strains were positive for Ahh gene .The Ahh genes of representative strains which were i-solated from different hosts shared 94.1%~98.9% nucleotide sequence homology with the reference strains . The Ahh genes of 7 representative strains showed a higher percentage of similarity (97.1%~100%).Among them, Ahh gene se-quence homologies of representative strains HC 960715-5 isolated from C.idellua and HTQ010505-3 isolated from W.pigra, as well as HL060811-3 isolated from M.piceus and HQ070610 B-3 isolated from R.chensinensis, were as high as 100%.
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