Correlation between {KCNQ1} and {KCNJ11} gene polymorphisms and type 2 and post-transplant diabetes mellitus in the Asian Indian population

摘要

Abstract Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) share a common pathophysiology. However, diabetes mellitus is a complex disease, and {T2DM} and {PTDM} have different etiologies. {T2DM} is a metabolic disorder, characterized by persistent hyperglycemia, whereas {PTDM} is a condition of abnormal glucose tolerance, with variable onset after organ transplant. The {KCNQ1} and {KCNJ11} gene encode potassium channels, which mediate insulin secretion from pancreatic β-cells, and {KCN} gene mutations are correlated with the development of diabetes. However, no studies have been carried out to establish an association between {KCNQ1} and {KCNJ11} gene polymorphisms and {T2DM} and PTDM. Therefore, our study was aimed at the identification of the role of {KCNQ1} and {KCNJ11} gene polymorphisms associated with {T2DM} and the risk of developing {PTDM} in the Asian Indian population. We have carried out a case–control study including 250 patients with T2DM, 250 control subjects, 42 patients with {PTDM} and 98 subjects with non-PTDM. PCR-RFLP analysis was carried out following the isolation of genomic {DNA} from EDTA-blood samples. The results of the present study reveal that two single nucleotide polymorphisms (rs2283228 and rs5210, of the {KCNQ1} and {KCNJ11} genes, respectively) are associated with both {T2DM} and PTDM. The results of our study suggest a role of {KCNQ1} and {KCNJ11} gene variants in the increased risk of {T2DM} and {PTDM} in the Asian Indian population.