Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

Yuanpeng Gao; Haibo Wu; Yongsheng Wang; Xin Liu; Linlin Chen; Qian Li; Chenchen Cui; Xu Liu; Jingcheng Zhang; Yong Zhang

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Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

机译：单个Cas9切口酶诱导的NRAMP1敲入牛产生，脱靶效应降低

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Abstract Epigenetic clocks provide powerful tools to evaluate nutritional, hormonal, and genetic effects on aging. What can we learn from differences between species in how these clocks tick?

机译：摘要表观遗传钟为评估营养，激素和遗传对衰老的影响提供了强大的工具。我们如何从物种之间的差异中了解到这些时钟如何滴答？

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《Genome Biology》 |2017年第1期|共15页
作者
Yuanpeng Gao; Haibo Wu; Yongsheng Wang; Xin Liu; Linlin Chen; Qian Li; Chenchen Cui; Xu Liu; Jingcheng Zhang; Yong Zhang;
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中图分类分子生物学;
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1. Cas9 Nuclease, Cas9 Nickaseを用いたoff-targetへの変異導入効率の比較 [J] . 三上雅史, 遠藤真咲, 土岐精一育种学研究 . 2015,第Suppla2期

机译：使用Cas9核酸酶和Cas9切口酶对脱靶突变引入效率的比较
2. Cas9 Nuclease, Cas9 Nickaseを用いたoff-targetへの変異導入効率の比較 [J] . 三上雅史, 遠藤真咲, 土岐精一育种学研究 . 2015,第Suppla2期

机译：CAS9核酸酶，Cas9酸氨基酶诱变效率与脱靶的比较
3. Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On- and Off-Target Damage Techniques for Detecting, Predicting, and Mitigating the On- and off-target Effects of Cas9 Editing [J] . Newman Anthony, Storrs Lora, Burgio Gaetan BioEssays : . 2020,第9期

机译：Cas9削减和后果; 检测，预测和减轻CRISPR / CAS9和偏离目标损坏技术，用于检测，预测和减轻CAS9编辑的开启和离心效果
4. SMART Libraries and Phage-Induced Directed Evolution of Cas9 to Engineer Reduced Off-Target Activity [C] . Derek Cerchione, Katie Loveluck, Eric Tillotson, Protein Engineering Summit. . 2018

机译：智能图书馆和噬菌体诱导的CAS9针对工程师的指向演化减少了偏离目标活动
5. Techniques to Increase Silage Stability and Starch Availability and the Effects of Heat Stress Abatement Systems on Reducing Heat Load in Dairy Cattle [D] . Johnson, Jared R. 2017

机译：提高青贮饲料稳定性和淀粉利用率的技术以及减轻热应激系统对降低奶牛热负荷的影响
6. Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects [O] . Yuanpeng Gao, Haibo Wu, Yongsheng Wang, 2017

机译：单个Cas9切口酶诱导的NRAMP1敲入牛产生脱靶效应降低
7. Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects [O] . Yuanpeng Gao, Haibo Wu, Yongsheng Wang, 2017

机译：单个Cas9切口酶诱导的NRAMP1敲入牛产生，脱靶效应降低

Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

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