Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer's disease and other neurodegenerative diseases

摘要

A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (n=39) and patients with other neurodegenerative disorders (n=38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; @e4 allele carriers had significantly lower apoE levels in blood than non-carriers.