Upregulation of LINC00982 inhibits cell proliferation and promotes cell apoptosis by regulating the activity of PI3K/AKT signaling pathway in renal cancer

摘要

OBJECTIVE: Previous studies have confirmed that lncRNAs are involved in the progression of multiple tumors. However, the role of lncRNAs in renal cancer remains unclear. Our study focused on investigating the expression and function of LINC00982 in renal cancer progression. PATIENTS AND METHODS: Quantitative Real time-polymerase chain reaction (qRT-PCR) assay was used to detect the expression of LINC00982 in renal cancer tissues and cell lines. LINC00982 expression was upregulated by transfecting with lentivirus. Western blot assay was used to detect the alteration expression levels of the relative protein involved PI3K/AKT signaling pathway. RESULTS: Downregulated LINC00982 expression was significantly observed in cancer samples when compared with the adjacent specimens, and was related to tumor size and TNM stage. Upregulation of LINC00982 inhibited proliferation and promoted apoptosis of ACHN and A-498 cell lines. LINC00982 could regulate the activity of PI3K/AKT signaling pathway. CONCLUSIONS: Upregulation of LINC00982 inhibits cell proliferation and promotes cell apoptosis by regulating the activity of PI3K/AKT signaling pathway in renal cancer.