miR-28 promotes cardiac ischemia by targeting mitochondrial aldehyde dehydrogenase 2 (ALDH2) in mus musculus cardiac myocytes

摘要

OBJECTIVE: Aldehyde dehydrogenase 2 (ALDH2) is a crucial enzyme involved in protecting the heart from ischemic. MicroRNAs (miRNAs) are involved in gene down-regulation. However, this mechanism is unclear. The aim of this study was to investigate the role of miR-28 in the regulation of ALDH2 and to explore the mechanism of miR-28 in musculus of myocardial ischemia. MATERIALS AND METHODS: To evaluate the role of miR-28, we assessed cellular apoptosis. In addition, the regulation of ALDH2 by miR-199b was evaluated by Western blotting and luciferase assay. RESULTS: MiR-28 was up-regulated, while ALDH2 expression decreased in a time-dependent manner under normoxic conditions. The miR-28-transfected cells showed a significant decrease in the cellular apoptosis. Compared with the negative control 1 precursor molecules, miR-28 over-expression caused about 55% increase in myocardial apoptosis under hypoxic conditions, and miR-28 silencing by anti-miR-28 attenuated a 41% decreasing in apoptosis. MiR-28 and pGL3-ALDH2 vector-transfected cells showed that ALDH2 protein expression was suppressed and luciferase activity was reduced. CONCLUSIONS: These findings suggest that miR-28 promotes myocardial ischemia through the inhibition of ALDH2 expression in mus. miRNAs is as a probable index in identification of myocardial ischemia after acute myocardial infarction.