Effects of metoprolol and small intestine RNA on marrow-derived endothelial progenitor cells applied for autograft transplantation in heart disease

摘要

AIMS: The objective of this project was to improve the effect of EPC autograft transplantation and observe the tolerance of EPCs to I/R injury affected by metoprolol and small intestine RNA. MATERIALS AND METHODS: We isolated bone marrow-derived EPCs and examined the effects of metoprolol and small intestine RNA on EPCs to ischemia at different time points after reperfusion. EPCs growth curve, secretion, apoptosis and mortality were also analyzed. RESULTS: EPCs will be better protected if the blood can be recovered within 4 hours after ischemia for cardiac muscle cells and pretreatment of EPCs with metoprolol or small intestine RNA could protect and promote EPCs proliferation. CONCLUSIONS: Our study demonstated that pretreatment of EPCs with metoprolol or small intestine RNA will increase the EPCs proliferation and may improve the EPCs autograft transplantation ability.