Objective To investigate the effect of bone narrow-derived endothelial progenitor cells (EPCs) on the intimal proliferation in a mouse model of transplant arteriosclerosis. Methods EPCs were isolated from bone marrow-derived mouse mononuclear cells (MNCs) by using a Ficoll density gradient centrifugation, and then were cultured in endothelial basal medium. To establish a model of transplant arteriosclerosis,a segment of abdominal aorta was transplanted orthotopically from C57BL/6 to BALB/c mice. Then,1× 107500 μ EPCs (n = 18) or 500 μL vehicle (0. 9% normal saline) was transplanted into the recipients byintravenous transfusion. The grafts were harvested at various time points after the operation, then endothelial regeneration rate and regional changes in the lumen and intimal were detected. Results The adherent cells were considered EPCs which both showed spindle shape and had many endothelial characteristics. Two weeks after transplantation,compared with control group, transplantation of EPCs markedly accelerated endothelium injury of allografts. Four weeks later, the neointimal thickness in transplantation group was significantly higher than that of control group. Conclusions EPCs may participate in the formation of the vascular lesion in aortic allografts. Bone marrow-derived EPCs promote endothelial damage and contribute to the neointima formation in a murine model of transplant arteriosclerosis.%目的 探讨输入受体骨髓源性内皮祖细胞(endothelial progenitor cell,EPC)对移植性动脉硬化的影响.方法 密度梯度离心法联合贴壁诱导法分离培养小鼠骨髓源性EPC,并对其进行鉴定.利用小鼠同种异体腹主动脉移植(以C57BL/6小鼠为供体,BALB/c小鼠为受体)建立移植性动脉硬化模型,术后通过尾静脉将体外培养的EPC输入模型体内,2周后观察移植动脉内膜损伤修复情况及病理改变；术后4周用计算机图像分析系统测量移植动脉新生内膜增生情况.结果 骨髓源性单个核细胞(mononuclear cell,MNC)体外可诱导培养出EPC,其细胞表面既表达干细胞标记,又表达内皮细胞特异性标记,并具有内皮细胞的生物学功能.小鼠腹主动脉移植术后2周,EPC输入组移植动脉内膜损伤较对照组明显加重.术后4周,EPC输入组移植动脉新生内膜厚度及管腔狭窄程度较对照组显著增加.结论 受体骨髓源性EPC参与移植动脉新生内膜形成.输入外源性EPC加重移植动脉内膜损伤,对移植性动脉硬化起促进作用.
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