IFN-γ induces upregulation of TNF-α, downregulation of MMP-2 and MMP-9 expressions in abortion rat

摘要

OBJECTIVE: Tumor necrosis factor-α (TNF-α) participates in the regulation of the whole process of pregnancy. Matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) play important roles in the process of trophoblast invading decidua. This research aims to determine the role of IFN-γ in TNF-α, MMP-2, and MMP-9 of abortion rats. MATERIALS AND METHODS: The rats were divided into control, abortion model, gestation, and IFN-γ group. Abortion rats model in IFN-γ group were treated by IFN-γ at low and high doses upon abortion model. Serum and tissue TNF-α, MMP-2, and MMP-9 expressions were detected by ELISA and immunohistochemistry. RESULTS: The level of TNF-α was significantly elevated, while MMP-2 and MMP-9 were statistically declined in the serum and decidual tissue of rat from model group (p 0.05). Of note, the expression of TNF-α was further increased, whereas MMP-2 and MMP-9 reduced in IFN-γ high dose group (p 0.05). CONCLUSIONS: We demonstrated that in abortion rats, TNF-α was overexpressed, while MMP-2 and MMP-9 were reduced declined in the serum and decidual tissue. The treatment with a high dose of IFN-γ further upregulated TNF-α expression and decreased MMP-2 and MMP-9 levels, aggravating the severity of rat abortion.