Evaluation of Ex-vivo Anti-arthritic,Anti-inflammatory, Anti-cancerous and ThrombolyticActivities of Mussaenda roxburghii Leaf

摘要

Aim: The current study evaluates the anti-arthritic, anti-inflammatory, anti-cancerous and thrombolytic activities of methanolic extract of Mussaenda roxburghii leaf.Place and Duration of study: This study was conducted in the Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, during the period between August 2013 and March 2014.Methodology: Bovine serum albumin was used for the evaluation of anti-arthritic potential and methanolic plant extract was compared with diclofnac sodium. The anti-inflammatory activity was assessed by hypotonicity induced membrane lysis and methanolic plant extract was also compared with diclofenac sodium. Brine shrimp lethality bioassay was used to evaluate the anti-cancerous activity of methanolic extract of Mussaenda roxburghii. Evaluation thrombolytic activity of methanolic extract of Mussaenda roxburghii was investigated by clot lysis method and methanolic extract was compared with streptokinase.Results: In the case of anti-denaturation, methanolic extract of Mussaenda roxburghii exhibited 42.15% of inhibition at 31.25 μg/ml and 80.33% of inhibition at 1000 μg/ml concentration. For anti-inflammatory effect, methanolic extract showed 53.79% and 86.93% of membrane stabilization activity at 31.25 μg/ml and 1000 μg/ml concentration respectively. The extract showed minimal anticancerous activity, lethal concentration (LC50) value of the extract was 282.03 μg/ml compared to vincristine sulphate (12.59 μg/ml). For the evaluation of the thrombolytic activity, methanolic leaf extract of Mussaenda roxburghii was treated with human blood. It showed promising activity which was about 49.09%.